Table 3.
Tissue phosphoproteomics.
| Study (year) | Tissue | Enrichment method | Quantitation | Identified sites | Ref. |
|---|---|---|---|---|---|
| Monetti et al. (2011) | Murine liver | SCX/TiO2 | Spike-in SILAC | up to 20,491 phosphorylation sites | [65] |
| Corradini et al. (2014) | Murine brain | Ti4+-IMAC | Manual inspection | 3690 identified 3271 quantified |
[67] |
| Lundby et al. (2012) | 14 rat organs and tissues | TiO2 | Label-free | Total 31,480 phosphorylation sites, 7280 proteins | [55] |
| Huttlin et al. (2010) | 9 murine tissues | SCX/ Fe3+-IMAC | Label-free | 35,965 phosphorylation sites, 6296 proteins | [21] |
| Lundby et al. (2013) | Murine hearts | TiO2 | Label-free | 8518 phosphorylation sites, 4246 proteins | [64] |
| Narumi et al. (2012) | Human breast cancer tissues | Fe3+-IMAC | iTRAQ SRM-based validation |
8309 phosphorylation sites on 3401 proteins 19 validated by SRM |
[68] |
| Wakabayashi et al. (2013) | Formaline-fixed, paraffin- embedded and fresh murine liver | HAMMOC | Chemical labeling based on reductive dimethylation | 1090 phosphopeptides | [62] |
Large-scale tissue-specific phosphoproteomics studies. Cell studies of phosphoproteomics listing: Biological context of the experiments, phosphopeptide enrichment method, quantitation method, number of identified sites and reference.
HAMMOC: Hydroxyl acid-modified metal oxide chromatography; iTRAQ: Isobaric tags for relative and absolute quantitation; SCX: Strong cation exchange; TiO2: Titanium dioxide.