Fig. 2.
Amblyomin-X increases intracellular calcium levels in SK-MEL-28 cells. a [Ca2+]i levels of unstimulated SK-MEL-28 and human fibroblast cells were measured for 20 s, followed by addition (marked by arrows) of Amblyomin-X (1 µM), TAPS (1 uM), or 50 uL of PBS 1X (Ctrl), and monitored for more than 100 s by microfluorimetry. b Graphic representation of quantitative measurements of fluorescence intensity (max–min) obtained from a. c Cells were treated with Amblyomin-X, stained with calcium green-1, and analyzed by flow cytometry. Representative histograms are expressed in fluorescence intensity. d Bars graph and geometric mean (fluorescence intensity) values ± SD obtained from C (three independent experiments). e Cells were pre-incubated for 30 min with BAPTA-AM (10 µM), followed by incubation with Amblyomin-X (1 µM) for 48 h at 37 °C