Fig. 4.

IL-6, IL-13 and IL-5 in lungs of HDM-allergic mice. IL-6 (a), IL-13 (c) concentrations were measured in supernatant of lung homogenates. IL-5 (d) was measured in the BALF. Correlation of IL-6 in lung homogenates and the total number of BALF cells (b), IL-5 in the BALF and the number of lymphocytes (e) and IL-5 in the BALF and the number of eosinophils (f). HDM–PBS: HDM-sensitized and PBS-challenged mice (white bars), HDM–HDM: HDM-sensitized and HDM-challenged mice (gray bars), Contr: control diet, GFBB: mixture of GOS and long-chain fructo-oligosaccharide with Bifidobacterium breve M-16V diet, FFBB: mixture of short-chain fructo-oligosaccharide and long-chain fructo-oligosaccharide with Bifidobacterium breve M-16V diet. Results are shown as mean ± SEM. Statistical significance of differences by one-way ANOVA and post hoc Bonferroni’s multiple comparisons test. *P < 0.05, **P < 0.01, ****P < 0.0001 compared to HDM–HDM; ^$$ P < 0.01, ^$$$ P < 0.001 compared to corresponding PBS group of diet, n = 5–9 mice/group. Correlation was analyzed using Spearman’s correlation test