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. Author manuscript; available in PMC: 2016 Apr 4.
Published in final edited form as: Virology. 2015 Dec 31;489:116–127. doi: 10.1016/j.virol.2015.12.005

Table 1.

φRIO-1 virion proteins detected by mass spectrometry.

kDaa % cov. Copies from gel densityb Proposed function Expected copiesc SC/MW Terminal residues unsampled (N,C)
gp37 174.9 65 4±2 IVP-D     4   4.3 0,0
gp55 73.2 86 12±6 Side fiber   18 11.2 6,0
gp36 63.8 70 6±3 IVP-C     8   7.8 0,0
gp51 63.6 68 9±4 Portal   12   6.2 0,0
gp40 44.4 78 7±3 Tube B (C ter)     6   3.6 7,5
gp42 41.4 63 23±15d   4.0 38,5
gp44 35.0 59 n.d.d Tube B (N ter)     6   0.9 5,0
gp48 35.2 97 415e Major capsid 415 53.5 0,0
gp46 28.8 49 15±7 Tube A   12   2.5 21,4
gp47 17.6 86 275±140f 14.6 0,0
gp43 19.1 75 n.d.f   5.6 1,4
gp38 14.8 79 <17g IVP-B   12   9.4 43,0
a

Molecular weight adjusted for loss of N terminal methionine confirmed by semitryptic fragment observed for gp37, gp36, gp51, and gp43. Presence of N-terminal methionine was confirmed for gp48 and gp47.

b

Number of polypeptides per virion with estimated 95% confidence interval.

c

From the precedent in T7 (Molineux, 2006; Guo et al., 2013).

d

Gp42 and gp44 comigrate; the sum is given.

e

Peak area of gp48 was assumed to correspond to 415 copies for calibration purposes.

f

Gp47 and gp43 comigrate; the sum is given.

g

There was no Coomassie-staining peak observed in the gel profile within the slice exhibiting gp38 tryptic fragments. The total area above our best estimate of the baseline across the slice was used to project the indicated maximum copy number.