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Flow cytometric analysis for pluripotent and lineage markers expressed on day 6 of differentiation in the presence of drugs. Alexa 647-APC and Alexa 488 antibodies were used to double-stain cells to look for pluripotency (Oct4+/Brachury-), mesendoderm (Oct4-/Bra+, Bra+/FoxA2-), endoderm (FoxA2+) and neurectoderm (NCAM+, NCAM+/nestin+). (A) Antibody controls using RA-treated cells. (B) Lineage-specific marker analysis.
