Fig. 2.

Real time RT–PCR for P2X₃ gene expression in the thoracic DRGs and NGs from 7D-ligated and sham-operated rats. a In DRGs, cDNAs from respective groups were amplified using primers for P2X₃. b In NGs, cDNAs from respective groups were amplified using primers for P2X₃. ΔC_T indicates the difference between the number of cycles necessary to detect the PCR products of the experimental gene and the reference gene. Ordinate values $(2_{\mathrm{T}}^{\mathrm{\Delta }C})$ correspond to the receptor mRNA expression relative to the reference gene in the tissue. Values are the mean ± SD of three real time PCR determinations