Fig 6. Two Specimens with Low-Allele Frequency FLT3 Internal Tandem Duplications.

In one specimen (A and B), a 24 bp internal tandem duplication (ITD) was seen in 7 out of 529 reads for an allele frequency of 1.3%. (A) Four of the reads containing insertions (purple bars) are shown using the Integrative Genomics Viewer. This specimen additionally harbored a FLT3 D839G mutation in 45% of reads (B). A second specimen (C) harbored a 33 bp FLT3 ITD in 12 out of 739 reads, for an allele frequency of 1.6%. Nine of the reads carrying an ITD are pictured.