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. 2016 Apr 4;11(4):e0152975. doi: 10.1371/journal.pone.0152975

Fig 3. The upper band is heteroduplex of wild type and mutant CCR5 delta 32.

Fig 3

(A) 3% agarose gel electrophoresis of PCR products are showed from Jurkat, HEK293T cells, wild type CCR5 plasmid (CCR5+/+), CCR5 delta 32 plasmid (CCR5 delta 32/delta 32), and the template mixture of wild type CCR5 and CCR5 delta 32 (CCR5/CCR5 delta 32). “NC” represents no template negative control. (B) 3% agarose gel electrophoresis of PCR products are indicated respectively from PCR amplification of wild type CCR5 plasmid (lane 1, CCR5 +/+), CCR5 delta 32 plasmid (lane 2, CCR5 delta 32/delta 32), and HEK293T cells (lane 5). Lane 3 shows the unheated PCR product mixture of wild type CCR5 and delta 32 (CCR5+CCR5 delta 32 mixture, samples from lane 1 and lane 2). Lane 4 shows the heated PCR product mixture of wild type CCR5 and delta 32 (Heated CCR5+CCR5 delta 32 mixture, samples from lane 1 and 2).