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. Author manuscript; available in PMC: 2017 Mar 1.
Published in final edited form as: Angew Chem Int Ed Engl. 2016 Feb 2;55(10):3369–3372. doi: 10.1002/anie.201510518

Figure 2.

Figure 2

(A–C, Top) Live-cell imaging of HeLa cells as a function of time after addition of 10 μM fluorescein-labeled CLIP6 peptide (scale bar = 10 μm). Associated z-stacks are also shown. (A–C, Bottom) Percentage of total cellular fluorescence measured at the membrane, within the cytoplasm or localized at the nucleus. Mechanism of CLIP6 (D) or TAT (E) uptake into A549, HeLa or OVCAR-3 cells was assessed after a 1 hour incubation with 10 μM of peptide alone (control, gray bars), under ATP depletion (open bars), or with hyperosmolar sucrose (black bars) preconditioning. Uptake results under ATP depletion and hyperosmolar sucrose conditions were compared to direct peptide incubation (gray bars) to determine statistical significance, denoted by * = p ≤ 0.05, or ** = p ≤ 0.01.