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. Author manuscript; available in PMC: 2017 Mar 1.
Published in final edited form as: Angew Chem Int Ed Engl. 2016 Feb 2;55(10):3369–3372. doi: 10.1002/anie.201510518

Figure 5.

Figure 5

(A) Ligand-mediated CreER activation in Rosa26-driven tdT/EGFP MEF cells. Delivery of a membrane-impermeable 4-OHCQ (yellow) small molecule by CLIP6 (blue) leads to CreER-mediated recombination at LoxP sites (→) and subsequent expression of EGFP. Images of untreated MEFs (B), or those incubated with the cell impermeable 4-OHCQ ligand (C), peptide alone (D), the CLIP6-4-OHCQ conjugate (E), or the 4-OHC membrane permeable positive control (F) (20× magnification, scale bar = 200 μm). Individual fluorescence channels used to compile the merged images can be found in Figure S7. (G) Flow cytometry quantification of the percentage of cells displaying tdT (red) or EGFP (green) intracellular fluorescence following treatment with the indicated compounds. Standard deviation in data shown is <1.5% for each treatment condition.