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. Author manuscript; available in PMC: 2016 Apr 4.
Published in final edited form as: Cell Rep. 2015 Aug 20;12(9):1367–1376. doi: 10.1016/j.celrep.2015.07.059

Figure 2. The Processing of CHL1 by BACE1 Is Required for Sema3A-Induced Growth Cone Collapse in Thalamic Neurons.

Figure 2

(A and B) Western blot analysis of the conditioned medium and cell lysates from WT thalamic neurons in response to 5 nM Sema3A-Fc.

(C and D) Quantification of the western blot analysis for soluble CHL1 (sCHL1) and APP C-terminal fragment β (C99).

(E) Schematic representation of BACE1 cleavage site on CHL1 (Gln1061/Asp1062).

(F and G) Analysis of Sema3A-induced growth cone collapse in Chl1−/− thalamic neurons transfected with empty vector, Fl-Chl1, and Fl-Chl1 and treated with 1 μM CIV or with non-cleavable Chl1D1062H.

Results are presented as mean ± SEM. The scale bars represent 5 μm. See also Figure S2.