Table 1.
Fluoxetine and ibuprofen mitigate LPS-induced sickness behavior in mice
| Parameters | Saline a | LPS a | SSRI a | NSAID a |
|---|---|---|---|---|
| Myeloperoxidase b | 0 ± 0 (3) | 8.26 ± 5.09 (3) c | 12.4 ± 8.12 (3) c | 0.34 ± 0.22 (3) |
| Body weight change (g) d | 0.6 ± 0.24 (3) | 3.77 ± 0.13 (5) c | 3.22 ± 0.12 (9) c,e | 3.79 ± 0.17 (7) c |
| Distance (m) f | 13.1 ± 1.21 (3) | 0.92 ± 0.06 (5) c | 1.29 ± 0.15 (9) c,e | 1.52 ± 0.21 (7) c,e |
| −93.0 | −90.2 | −88.4 | ||
| Number of moves (# × 100) f | 21.0 ± 0.55 (3) | 3.89 ± 0.14 (5) c | 5.07 ± 0.80 (9) c,e | 6.52 ± 0.96 (7) b, e |
| −81.5 | −75.9 | −69.0 | ||
| Move time (min) f | 96.4 ± 4.83 (3) | 13.0 ± 0.48 (5) c | 17.2 ± 2.40 (9) c,e | 22.17 ± 3.13 (7) c,e |
| −86.5 | −82.2 | −77.0 |
The mice received either i.p. injections of saline, fluoxetine (10mg/Kg), or ibuprofen (10 mg/Kg) every day for three days and then LPS (5mg/Kg) or saline on the fourth day. Twenty four h later the animals were sacrificed and their brains collected for the measurement of MPO. The sickness behavior measurements are depicted on a grey background. Shown are the means ± SEM (n) values and the percent changes in italics.
Values represent the density measurement of myeloperoxidase immunoreactivity as determined by Western blotting and normalized against the β-actin density in each blot.
Different from Saline group, p <0.05 (ANOVA with Kruskal Wallis post hoc analysis).
’Change in weight’ reflect the difference in weight measured just prior the LPS injection and 24 h later at the time of sacrifice.
Different from LPS group, p <0.05 (ANOVA with Kruskal Wallis post hoc analysis).
The behavioral measurement were recorded between 20 and 24 hour post LPS (4 h).