Table 2. OAW and nutrients effects on growth and survival.
Results from split-plot ANOVA with the fixed factors ‘OAW’ and ‘Nutrients’ and the random factors ‘Mesocosm’ and ‘Sibling group’. Effects are shown for (A) growth rates (% d-1) and (B) survival during the OAW x nutrient experiment and (C) survival during the upwelling experiment. Df: degrees of freedom, SS: sums of squares and MS: mean squares. ‘Den. Syn. Error df’ and ‘Den. Syn. Error MS’ describe the denominator synthesis of degrees of freedom and mean squares, respectively. (Datasets of area and growth values can be found in the PANGAEA dataset).
| Source of variation | df | SS | MS | Den. Syn. Error df | Den. Syn. Error MS | F-value | p-value |
|---|---|---|---|---|---|---|---|
| (A) Growth rates OAW x nutrient experiment | |||||||
| OAW | 1 | 47.659 | 47.659 | 6.156 | 1.188 | 40.111 | < 0.001 |
| Nutrients | 1 | 20.468 | 20.468 | 8.469 | 1.428 | 14.335 | < 0.05 |
| OAW x Nutrients | 1 | 13.473 | 13.473 | 8 | 1.339 | 10.055 | 0.013 |
| Mesocosm (OAWxNutrients) | 8 | 10.720 | 1.340 | 135 | 0.389 | 3.443 | 0.001 |
| Sibling | 15 | 27.120 | 1.808 | 5.276 | 0.325 | 5.558 | 0.030 |
| Sibling x OAW | 15 | 3.561 | 0.237 | 135 | 0.389 | 0.610 | 0.863 |
| Sibling x Nutrient | 15 | 7.156 | 0.477 | 135 | 0.389 | 1.226 | 0.260 |
| Error | 135 | 52.542 | 0.389 | ||||
| (B) Survival OAW x nutrient experiment | |||||||
| OAW | 1 | 4.069 | 4.069 | 14.366 | 0.867 | 4.694 | 0.048 |
| Nutrients | 1 | 2.903 | 2.903 | 7.061 | 0.513 | 5.656 | 0.049 |
| OAW x Nutrients | 1 | 1.272 | 1.272 | 8 | 0.535 | 2.376 | 0.162 |
| Mesocosm (OAWxNutrients) | 8 | 4.281 | 0.535 | 135 | 0.163 | 3.284 | 0.002 |
| Sibling | 15 | 11.786 | 0.786 | 12.535 | 0.473 | 1.662 | 0.186 |
| Sibling x OAW | 15 | 7.420 | 0.495 | 135 | 0.163 | 3.036 | < 0.001 |
| Sibling x Nutrient | 15 | 2.117 | 0.141 | 135 | 0.163 | 0.866 | 0.603 |
| Error | 135 | 21.998 | 0.163 | ||||
| (C) Survival Upwelling experiment | |||||||
| OAW | 1 | 4.907 | 4.907 | 8.398 | 0.550 | 8.914 | 0.017 |
| Nutrients | 1 | 0.322 | 0.322 | 5.284 | 0.423 | 0.761 | 0.421 |
| OAW x Nutrients | 1 | 2.913 | 2.913 | 8 | 0.516 | 5.647 | 0.045 |
| Mesocosm (OAWxNutrients) | 8 | 4.127 | 0.516 | 135 | 0.164 | 3.150 | 0.003 |
| Sibling | 15 | 10.711 | 0.714 | 3.504 | 0.105 | 6.793 | 0.052 |
| Sibling x OAW | 15 | 2.976 | 0.198 | 135 | 0.164 | 1.212 | 0.270 |
| Sibling x Nutrient | 15 | 1.057 | 0.070 | 135 | 0.164 | 0.430 | 0.968 |
| Error | 135 | 22.106 | 0.164 | ||||