Fig. 6.

Pattern dimensions determined the preferred focal adhesion orientations. Parallel focal adhesions were present on substrates with pitches of 800, 1200, 1600, and 2000 nm but not on 400 and 4000 nm pitch substrates. Obliquely oriented focal adhesions extended over multiple grooves on 400, 800, and 1200 nm pitch but were confined to single ridges on 1600, 2000, and 4000 nm pitch substrates. Percentages are relative to the total number of cells that formed focal adhesions.