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. Author manuscript; available in PMC: 2017 Jan 1.
Published in final edited form as: Mol Neurobiol. 2014 Dec 11;53(1):595–610. doi: 10.1007/s12035-014-9032-y

Fig. 2.

Fig. 2

TRPM7 knockdown with shRNA increases total neurite outgrowth and length of the longest neurite. a Representative confocal image overlays (top panel) and eGFP signal (bottom panel) of DIV4 hippocampal neurons infected with different AAV-1 constructs. b Total neurite length and c total number of neurites per neuron. For groups at DIV4: eGFP only n=42, scrambled shRNA n=43, TRPM7 shRNA n=48; for groups at DIV6: eGFP only n=26, scrambled shRNA n=40, TRPM7 shRNA n=35; for groups at DIV8: eGFP only n=25, scrambled shRNA n=20, TRPM7 shRNA n=23. Data are shown as mean±SEM. Statistical analysis: one-way ANOVA with Bonferroni post hoc; *p<0.05. Scale bars are 20 μm