TRPM7 knockdown with shRNA increases total neurite outgrowth and length of the longest neurite. a Representative confocal image overlays (top panel) and eGFP signal (bottom panel) of DIV4 hippocampal neurons infected with different AAV-1 constructs. b Total neurite length and c total number of neurites per neuron. For groups at DIV4: eGFP only n=42, scrambled shRNA n=43, TRPM7 shRNA n=48; for groups at DIV6: eGFP only n=26, scrambled shRNA n=40, TRPM7 shRNA n=35; for groups at DIV8: eGFP only n=25, scrambled shRNA n=20, TRPM7 shRNA n=23. Data are shown as mean±SEM. Statistical analysis: one-way ANOVA with Bonferroni post hoc; *p<0.05. Scale bars are 20 μm