Table 1.
Table of factors interaction
| 2-way ANOVA factor | Transporter | Brain structure FC | HIP | DS | |||
|---|---|---|---|---|---|---|---|
| Expression level mRNA | Protein | mRNA | Protein | mRNA | Protein | ||
| CEF | |||||||
| Pretreatment × surgery | GLT-1 | F(1,28) = 3.09, p = 0.089 | F (1,28) = 8.36, p = 0.007 | F(1,28) = 0.15, p = 0.702 | F (1,28) = 9.05, p = 0.005 | F (1,28) = 23.35, p < 0.001 | F (1,28) = 20.20, p < 0.001 |
| Pretreatment × surgery | xc- | F (1,28) = 5.5, p = 0.026 | F (1,28) = 13.95, p < 0.001 | F(1,28) = 0.63, p = 0.432 | F(1,28) = 1.61, p = 0.215 | F (1,28) = 33.41, p < 0.001 | F(1,28) = 1.98, p = 0.171 |
| NAC | |||||||
| Pretreatment × surgery | GLT-1 | F(1,28) = 1.82, p = 0.188 | F (1,28) = 7.08, p = 0.013 | F(1,28) = 0.09, p = 0.766 | F (1,28) = 13.68, p < 0.001 | F(1,28) = 0.09, p = 0.758 | F (1,28) = 4.65, p = 0.04 |
| Pretreatment × surgery | xc- | F (1,28) = 25.57, p < 0.001 | F(1,28) = 3.29, p = 0.08 | F(1,28) = 0.29, p = 0.594 | F(1,28) = 0.005, p = 0.944 | F(1,28) = 0.19, p = 0.66 | F (1,28) = 8.54, p = 0.007 |
| 3NP | |||||||
| Pretreatment × surgery | GLT-1 | F (1,28) = 14.39, p < 0.001 | F(1,28) = 2.95, p = 0.097 | F (1,28) = 8.89, p = 0.006 | F (1,28) = 10.11, p = 0.004 | F(1,28) = 1.41, p = 0.245 | F(1,28) = 2.47, p = 0.127 |
| Pretreatment × surgery | xc- | F (1,28) = 31.75, p < 0.001 | F(1,28) = 3.03, p = 0.093 | F (1,28) = 24.06, p < 0.001 | F(1,28) = 0.25, p = 0.62 | F(1,28) = 2.74, p = 0.109 | F(1,28) = 0.16, p = 0.689 |
| IP | |||||||
| Pretreatment × surgery | GLT-1 | F (1,28) = 24.52, p < 0.001 | F (1,28) = 30.76, p < 0.001 | F (1,28) = 39.49, p < 0.001 | F(1,28) = 3.63, p = 0.067 | F(1,28) = 1.94, p = 0.174 | F(1,28) = 0.293, p = 0.592 |
| Pretreatment × surgery | xc- | F (1,28) = 45.62, p < 0.001 | F(1,28) = 3.71, p = 0.064 | F (1,28) = 70.93, p < 0.001 | F(1,28) = 0.11, p = 0.741 | F(1,28) = 0.34, p = 0.564 | F(1,28) = 0.27, p = 0.606 |
Interactions between factors: pretreatments (NAC, CEF, 3NP or IP) and surgery (SHAM or ISCH)
NAC N-acetylcysteine, CEF ceftriaxone, 3NP 3-nitropropionic acid, IP ischemic preconditioning, GLT-1 Glu transporter-1, xc- Glu/cystine antiporter
F values, which reached statistical significance (shown in bold) indicate groups classified for further Post hoc analysis (Tukey’s test)