TABLE 3.

GMMA characterization^a

Strain	GMMA diam (nm)b		LPS content (nmol/mg GMMA)c	FliC equivalents (μg/mg GMMA)d
	Range (min–max)	Mean
STmG	27–80	47	173	0.55
STmG ΔhtrB	27–87	39	165	0.74
STmG ΔmsbB	20–60	42	155	0.65
STmG ΔmsbB ΔpagP	27–107	51	160	0.22
SEnG	27–73	45	157	6.99
SEnG ΔhtrB	27–107	47	100	1.35
SEnG ΔmsbB	33–80	47	164	1.49
SEnG ΔmsbB ΔpagP	27–80	44	529	3.69
SEnG ΔmsbB ΔhtrB	27–73	40	154	1.44

^aPerformed with single batches of GMMA purified from the different mutants.

^bFor 20 GMMA per type. min, minimum; max, maximum.

^cMeasured in duplicate per batch by quantification of KDO, with a coefficient of variance between the duplicate measurements of <5%.

^dMonomeric flagellin was quantified using the TLR5-specific assay in comparison to a FliC standard and was expressed as FliC equivalents. The coefficient of variance for 4 independent experiments was approximately 30%. Means are shown.