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. Author manuscript; available in PMC: 2016 Aug 1.
Published in final edited form as: Biotechnol Bioeng. 2015 Sep 3;113(2):414–423. doi: 10.1002/bit.25710

Figure 1.

Figure 1

Testing a miniaturized assay using an ideal LAB/AMP system. L. lactis NZ9000 containing plasmid to produce either LacZ (n = 62; white) or a combination of three enterocins (entA, enterocin P, and hiracin JM79; n = 10; gray) were randomly seeded into a 96-well plate. After LAB growth and peptide production, the cell-free supernatant was collected and applied (as 1% v/v) to 106 CFU/mL E. faecium 8 and fresh BHI medium. Pathogen cell density was measured as OD600 at 10, 12, 14, and 16 h. The data are shown in a box and whisker plot where the mean, first quartile, third quartile, standard deviation, and extrema are represented by the centerline, bottom line, top line, whiskers, and X’s, respectively.