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. Author manuscript; available in PMC: 2016 Aug 1.
Published in final edited form as: Biotechnol Bioeng. 2015 Sep 3;113(2):414–423. doi: 10.1002/bit.25710

Figure 5.

Figure 5

EntA and derivatives tested against multiple pathogens. (a) A24G, A24P, and T27G constructs were tested against multiple species of E. faecalis using a stab-on agar test. BHI with 0.8% agar and 0.5 μL/mL (E. faecalis) or 0.75 μL/mL (E. faecium) were allowed to solidify. Five colonies of L. lactis containing each construct were stabbed on to agar containing pathogen and allowed to grow at 37°C overnight. (b) The average halo diameter was measured using ImageJ. A one-tailed Student’s t-test compared mutants to entA. *indicates P≤0.05, and **indicates P≤0.01.