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. 2016 Apr 5;7:441. doi: 10.3389/fpls.2016.00441

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Copyright © 2016 Jin, Huang, Li, Yin, Li, Yao and Zhang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Overexpression of PubHLH1 enhances cold tolerance in transgenic tobacco. (A) Phenotypes of 30-day-old seedlings of WT and transgenic plants (OE4 and OE9) subjected to chilling treatment (0°C) for 18 h, followed by recovery at the normal growth temperature for 10 days. (B) Survival rates of the WT and transgenic plants after recovery from chilling stress. The survival rate is the ratio of the number of living plants after recovery from the chilling treatment to the total number of plants tested. (C) Phenotypes of 60-day-old WT and transgenic plants (OE4 and OE9) before and after chilling stress, followed by recovery for 15 days. (D–G) MDA content, electrolyte leakage, chlorophyll, and proline contents, respectively. Fresh weight (FW). Data represent the means ± SE of at least three replicates. Significant differences between WT and transgenic plants are indicated by asterisks (^∗∗P < 0.01, ^∗∗∗P < 0.001).