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Histochemical staining via trypan blue, nitro blue tetrazolium (NBT), and 3,3′-diaminobenzidine (DAB) to analyze cell death, and the accumulation of O2- and H2O2 in the transgenic (OE4 and OE9) and WT plants. (A) Representative photographs show the staining of cell death in the leaves before (upper panel) and after the chilling treatment. (B,C) The in situ accumulation of O2- and H2O2 in the transgenic and WT plants before and after the chilling stress.
