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. 2016 Mar 23;5(3):e23. doi: 10.1038/emi.2016.20

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Redistribution of GRP78/BiP was independent of the expression of KDEL receptor. (A) RD cells were infected with EV71 (MOI of 10), followed by whole-cell lysate preparation for western blot analysis of the expression levels of KDELR. The viral protein expression is indicated by the levels of viral protein 3D. (B) RD cells were transfected with KDELR-HA or vector control for 24 h and then infected with EV71 (MOI of 10). Cell homogenates were collected at 6 h p.i. and subjected to cytosolic/microsomal fractionations and western blot analysis for the distribution of GRP78/BiP. The results are representative of three independent experiments. The expression of KDELR-HA was detected with anti-HA antibodies (bottom panel).