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. 2016 Mar 23;5(3):e23. doi: 10.1038/emi.2016.20

Figure 6.

Figure 6

dsRNA induced a PKR-dependent cytosolic elevation of GRP78/BiP on EV71 infection. (A) EV71 infection and poly(I:C) transfection led to the phosphorylation of PKR, p38 MAPK and JNK. RD cells were infected with EV71 (MOI of 10, 5 and 6 h, respectively) or transfected with poly(I:C) for 18 h. Cells were lysed for western blotting with antibodies against the indicated proteins. Antibodies against total PKR, total p38 MAPK, total JNK and GAPDH were used to monitor loading. An asterisk marks a nonspecific band. (B–D) RD cells were infected with EV71 (MOI of 10) or mock-infected and subsequently treated with DMSO, SP600125 (20 μM), SB203580 (20 μM) or PKRi (10 μM) at 3 h p.i. The PNS was collected at 6 h p.i. to examine the levels of p-JNK, p-p38 MAPK and p-PKR by western blotting (left) or subjected to cytosolic/microsomal fractionations to analyze the distribution of GRP78/BiP with a western blot (right). An asterisk marks nonspecific bands. These results are representative of three independent experiments.