Figure 8.

EV71-induced redistribution of GRP78/BiP conferred prolonged ER stress and was advantageous to virus infection. (A–C) RNAi rescue experiments were performed by transfecting GRP78/BiP-knockdown RD cells with siRNA-resistant BiP_WT or BiP_ΔKDEL. The cells were then infected with EV71 (MOI of 10, 6 h p.i.). The viral protein expression, as indicated by the 3D level, was assessed by a western blot analysis (A), and the virus titers were determined by plaque assays (B). The plaque assay results are expressed as the means±s.d. (n=3). ***P<0.001 compared with the re-expression of vector alone. (C) The cells were treated with 2.5 mM DTT for 4 h, and the cell lysates were then collected to examine the levels of p-eIF2α with a western blotting analysis. These results are representative of three independent experiments.