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. 2015 Jun 29;35(13):1692–1702. doi: 10.1038/onc.2015.234

Figure 1.

Figure 1

HMG domain mediates SOX6-induced suppression of cell proliferation via cell cycle arrest in the G0/G1 phase. (a) Schematic of SOX6 and SOX6ΔHMG (HMG domain deletion) depicting the functional domain. (b) Ectopic expression of SOX6 or SOX6ΔHMG was measured in HeLa cells transfected with pLex-HA-SOX6 or pLex-HA-SOX6ΔHMG vectors. Proliferation of HeLa (c) or SMMC7721 (d) cells transfected with pLex-HA-SOX6, pLex-HA-SOX6ΔHMG or control expression vector, or of HeLa-tet cells (e) was measured using MTT assay. For the HeLa-tet cells, each cell type was cultured in medium with Dox (2 μg/ml) or control medium for 96 h. Data (c–e) shown are the mean±s.d. of 5 independent experiments (*P<0.05, **P<0.01 and ***P<0.001, Student's t-test, two-sided). The column diagram of the cell cycle of HeLa (f) or SMMC7721 (g) cells transfected with pLex-HA-SOX6, pLex-HA-SOX6ΔHMG or control expression vector, or of HeLa-tet cells (h), was measured using flow cytometry. Data (f–h) shown are the mean±s.d. of 5 independent experiments (*P<0.05, χ2 test, two-sided). The statistical tests (c–h) are justified as appropriate and meet the assumptions of the tests. The variance between the groups is similar. aa, Amino acids.