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. 2016 Apr 5;6:23892. doi: 10.1038/srep23892

Figure 2. UCA1 promotes CRC cell growth in vitro and in vivo.

Figure 2

(a,b) Effect of UCA1 on cell growth was detected by CCK-8 assay in HCT116/HT29 cells with silenced UCA1 and SW480/LoVo cells with overexpressed UCA1. (c) Effect of UCA1 knockdown and overexpression on colony formation of CRC cells. (d) Effect of UCA1 downregulation and overexpression on tumor growth in a xenograft mouse model. Overexpression of UCA1 promoted tumorigenesis, whereas silencing of UCA1 inhibited tumor growth. (e) HCT116 and SW480 cells transfected with siUCA1 or pWPXL-UCA1 were treated with 0 to 50 μg/mL 5-FU for 48 h, and cell viability was determined by CCK-8 assay. (f) Apoptosis of HCT116 and SW480 cells transfected with siUCA1/siNC or pWPXL-UCA1/pWPXL and treated with or without 0.2 μg/mL 5-FU was examined by flow cytometry. Representative scatter plots of PI vs Annexin V-FITC staining in one independent experiment. The values obtained from Annexin V assays represent the means ± S.D. for three independent experiments. *P < 0.05, **P < 0.01.