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. 2016 Apr 5;6:23921. doi: 10.1038/srep23921

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(a) The weight of the 5-HT_3A-ICD monomer construct was determined to be 53 kDa using SDS-gel electrophoresis. Molecular weight standards, top, 5-HT_3A-ICD, bottom. (b,c) Size-exclusion chromatography (SEC) yielded a weight of 265.1 kDa for 5-HT_3A-ICD. Chromatogram of standards, black, blue dextran, blue, and 5-HT₃ _A-ICD sample, red. (d) SEC-MALS confirmed pentameric assembly with the weight-average molar mass of 5HT_3A-ICD as 250.8 ± 4.4 kDa. A representative SEC-MALS profile showing the Rayleigh ratio (green line) and the weight-average molar mass (red line) computed from light scattering.