Table 2. Helicobacter pylori strains and plasmids used in this study.
| Strain or plasmid | Relevant characteristics | Source or reference |
|---|---|---|
| H. pylori strains and short designations | Strain characteristics and full designations | |
| N6 | parental strain; human patient isolate | 79 |
| N6 tlpD | H. pylori N6 tlpD::aphA3 | 26 |
| HP87P7 | wild type, gerbil-adapted | 13 |
| HP87P7 tlpD | H. pylori HP87 P7 tlpD::aphA3 | 13 |
| N6 tlpD (TlpD-Hisx6) | H. pylori N6 tlpD::aphA3 (pHel2::tlpD-hisx6) | this study |
| N6 tlpD (pHel2::tlpD-V5) | H. pylori N6 tlpD::aphA3 (pHel2::tlpD-V5) | 26 |
| N6 rdxA::tlpD-V5 | H. pylori N6 rdxA::tlpD-V5 CmR (chromosomal complementation) | this study |
| N6 tlpD rdxA::tlpD-V5 | H. pylori N6 tlpD::aphA3 rdxA::tlpD-V5 CmR | this study |
| N6 cheAY2 rdxA::tlpD-V5 | H. pylori N6 cheAY2::aphA3 rdxA::tlpD-V5 CmR | this study |
| N6 tlpABC rdxA::tlpD-V5 | H. pylori N6 tlpA::aphA3 tlpBC (unmarked partial deletions) rdxA::tlpD-V5 CmR | this study |
| N6 katA rdxA::tlpD-V5 | H. pylori N6 katA::aphA3 rdxA::tlpD-V5 CmR | this study |
| N6 acnB rdxA::tlpD-V5 | H. pylori N6 acnB::aphA3 rdxA::tlpD-V5 CmR | this study |
| N6 acxC rdxA::tlpD-V5 | H. pylori N6 acxC::aphA3 rdxA::tlpD-V5 CmR | this study |
| N6 (AcnB-V5) | H. pylori N6 (pHel2::acnB-V5) CmR | this study |
| N6 acnB | H. pylori N6 acnB::aphA3 | this study |
| N6 katA | H. pylori N6 katA::aphA3 | this study |
| N6 acxC | H. pylori N6 acxC::aphA3 | this study |
| N6 cheAY2 | H. pylori N6 cheAY2::aphA3 | 26 |
| N6 (NifSU+) | H. pylori N6 (pHel2::nifSU) | this study |
| Plasmids | Plasmid characteristics | |
| pUC18 | AmpR, RepEc; high-copy-number vector | 86 |
| pUT18 | AmpR; RepEc; pBR322 derivative; cloning vector | 87 |
| pILL570 | SpecR; RepEc; cloning vector | 88 |
| pEF6-V5 | AmpR, BlastR; RepEc; source of V5-tag sequence | Invitrogen Life Technologies, Darmstadt, Germany |
| pET28a | KmR; RepEc; high-copy-number expression vector, includes hexa-histidine tag sequence | Novagen |
| pHel2 | CmR; RepEc, RepHp; multi-copy-number vector; H. pylori expression plasmid | 89 |
| pKSF | KmR; RepEc; source of the kan-sacB construct | 90 |
| pCJ522 | CmR; tlpD-V5 fusion gene including the potential tlpD promoter region in pHel2 shuttle vector | 26 |
| pCJ545 | CmR; tlpD-hisx6 fusion gene including the potential tlpD promoter region in pHel2 shuttle vector | this study |
| pCJ1341 | KmR; pET28a derivative; tlpD fused N terminally with Hisx6 | this study |
| pCJ542 | AmpR, CmR; pUC18 derivative; tlpD-V5 expression construct and CAT cassette inserted in rdxA flanking regions (for chromosomal complementation) | 13 |
| pCJ1306 | AmpR, KmR; pUT18 derivative; acnB disrupted by aphA3 cassette | this study |
| pCJ1308 | AmpR, KmR; pUT18 derivative; katA disrupted by aphA3 cassette | this study |
| pCJ537 | AmpR, KmR; pUC18 derivative; acxC disrupted by aphA3 cassette | this study |
| pCJ513 | SpecR, KmR; pILL570 derivative; tlpB disrupted by kan-sacB construct (kanamycin resistant, sucrose sensitive) | this study |
| pCJ514 | SpecR; pILL570 derivative; tlpB partially deleted (unmarked) | this study |
| pCJ1396 | AmpR, KmR; pUC18 derivative; tlpA disrupted by aphA3 cassette | this study |
| pCJ1314 | CmR; acnB-V5 construct and potential acnB promoter region in pHel2 shuttle vector | this study |
| pCJ1350 | CmR; nifSU gene cluster including the potential promoter region in pHel2 shuttle vector | this study |
AmpR ampicillin resistance, KmR kanamycin resistance, CmR chloramphenicol resistance, BlastR blasticidin resistance, SpecR spectinomycin resistance, RepEc replication in E. coli, RepHp replication in H. pylori.