Table 4. Growth inhibition of H. pylori N6 and HP87 P7 wild type, tlpD mutant and tlpD complemented strains in agar diffusion assays with different concentrations of the iron chelator 2,2-dipyridyl (DP).
| Diameter of zone with growth inhibition [mm]a,b | ||||
|---|---|---|---|---|
| Strain | 10 mM 2,2-DP | Significance (p value) | 20 mM 2,2-DP | Significance (p value) |
| N6 wild type | 9.6 ± 0.5 | 15.8 ± 1.0 | ||
| N6 tlpD | 11.9 ± 0.9 | 0.017 | 17.8 ± 0.6 | 0.015 |
| N6 tlpD rdxA::tlpD-V5 | 10.0 ± 0.0 | 0.205 (ns) | 16.9 ± 0.3 | 0.118 (ns) |
| HP87P7 wild type | 8.2 ± 0.2 | 16.1 ± 0.4 | ||
| HP87P7 tlpD | 9.9 ± 0.7 | 0.029 | 18.3 ± 1.0 | 0.010 |
| HP87P7 tlpD rdxA::tlpD-V5 | 10.1 ± 1.3 | 0.138 (ns) | 17.7 ± 1.5 | 0.094 (ns) |
amean diameter of growth inhibition combined from duplicate experiments and standard deviation.
bp values for significance of difference calculated by Student’s t test in comparison to respective wild type.
ns: not significant.