Figure 6. Cellular activity of cjoc42 (abbreviated to ‘42’).

In (a,b) the left-hand figures show representative images and the right-hand figures show densitometry analysis of band intensities (n = 3 and SEM). All data were normalized to untreated mock-transfected cells, and all statistical comparisons (t tests) were made to these. ns, non-significant; *p < 0.05; **p < 0.01; ***p < 0.001; (a) Effects of gankyrin over-expression on levels of p53 and β-actin in U2OS cells 48 hours post transfection with 2 μg gankyrin plasmid, p53 expression levels are reduced by ~40% compared with control cells. cjoc42 (abbreviated to ‘42’) addition at the time of transfection inhibits this decrease in p53 levels. (b) Cells treated with cjoc42 alone do not show any changes in p53 levels compared to mock transfections. (c) Reduction of p53 luciferase reporter activity in gankyrin-overexpressing U2OS cells can be inhibited by cjoc42 (abbreviated to ‘42’) in a dose-dependent manner. cjoc42 alone at equivalent doses does not alter the luciferase reporter activity.