Figure 7. cjoc42 (abbreviated to ‘42’) inhibits gankyrin-induced decrease in p53 downstream target expression and resensitises cells to DNA damage.

(a) Four hours after DNA damage with Etoposide a reduction of p21 upregulation is seen in gankyrin-overexpressing U2OS cells (lane 2) compared to mock transected cells (lane 1). This can be prevented by cjoc42 addition (lane 3). cjoc42 alone shows upregulation of p21 (in the presence of DNA damage) to levels comparable with mock-transfected cells (lane 4). (b) Cell viability (expressed as a percentage of control) 24 hours after Etoposide treatment. Gankyrin-overexpressing U2OS cells incubated with cjoc42 show a dose-dependent loss of viability. Cells not expressing gankyrin (— Gankyrin) show no such dose-dependent change in cell viability.