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. 2016 Apr 5;6:24064. doi: 10.1038/srep24064

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Proteins derived from lower chamber of Transwell (or basolateral compartment of polarized cells) with equal amount of 80 μg were resolved by 2-DE and visualized with Deep Purple staining. Spot matching and intensity analysis revealed significant differences in levels of six protein spots (labeled with spot numbers) between the controlled and COM-treated groups. These proteins were then identified by Q-TOF MS/MS analysis (see details in Table 1). N = 5 independent gels in each group (a total of 10 gels were analyzed).