Figure 4.

Induction of EMT in SKOV-3 by elastase or PMN lysate: A Cytokeratin (upper panel) was determined in the cell lysates of untreated SKOV-3 or of SKOV-3 being exposed to either PMN elastase or PMN lysate. The antibody recognized three cytokeratins, expression of which declined in treated cells. With β-actin as loading control, it was seen that the density of the cytokeratin bands declined in treated cells (the numbers indicate the relative density obtained for the 55 kD band. Data of one of three experiments is shown). B In SKOV-3 treated with PMN lysate, a translocation into the nucleus of β-catenin was seen. As loading control the nuclear antigen p84 was used (one of three experiments is shown).