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. 2016 Mar 18;7(5):600–607. doi: 10.7150/jca.13820

Figure 1.

Figure 1

Down-regulation of Tie2 expression in OSCC-derived cell lines. (A) Quantification of Tie2 mRNA expression in OSCC-derived cell lines by qRT-PCR analysis. Significant down-regulation of Tie2 mRNA is seen in nine OSCC-derived cell lines compared with HNOKs (*p < 0.05, Mann-Whitney U-test). Data are expressed as the means ± standard errors of the mean of triplicate results. (B) Immunoblot analysis of Tie2 protein in OSCC-derived cell lines and HNOKs. Tie2 protein expression is down-regulated in OSCC-derived cell lines compared with HNOKs. Densitometric Tie2 protein data are normalized to GAPDH protein levels. The values are expressed as a percentage of HNOKs.