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. 2016 Apr 5;6:23989. doi: 10.1038/srep23989

Figure 2. DUF283 binds single-stranded nucleic acids of different lengths in a concentration-dependent manner.

Figure 2

Native PAGE gels showing the results of binding assays involving DUF283 and (a) 12-nt ssRNA, (b) 22-nt ssRNA, (c) 32-nt ssRNA, (d) 52-nt ssRNA, (e) 62-nt ssRNA. [C-] denotes controls with no DUF283. Triangles represent increasing amounts of DUF283 (0.5; 4.0; 8.0 μM). (f) DUF283 binds 22-nt ssRNA with Kd = 9.5 ± 0.5 μM. Native PAGE gel showing the results of binding assay involving DUF283 and R22 (left). [C-] denotes a control with no DUF283. A triangle represents increasing amounts of DUF283 (0.5; 1; 5; 10; 15; 20.0 μM). A binding curve of DUF283 and 22-nt ssRNA (right). The curve was derived from densitometric quantification of the autoradiogram. The Kd values were calculated from the curves and presented results are the mean of three independent assays. (g) Native PAGE gel showing the results of binding assays involving DUF283 and 29-nt ssDNA.