Figure 5. hDicer accelerates annealing of complementary oligonucleotides.

Native PAGE gels showing the results of annealing reactions involving hDicer and the following nucleotide pairs: (a) R22 and cR22, (b) cR22 and cR21, (c) D29 and cD29. Reaction mixtures were incubated for 30 min with increasing amounts of hDicer or with no protein [C-] and were then resolved in buffer containing SDS at a final concentration of 0.2%. [C^*] denotes the single-strand control containing the ³²P-labeled oligomer.