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. 2016 Apr 5;6:23989. doi: 10.1038/srep23989

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Copyright © 2016, Macmillan Publishers Limited

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Graphic presentation of the results obtained from three independent annealing assays involving DUF283 (left) or hDicer (right) and three oligonucleotide pairs, as follows: (a) R22 and cR22; (b) cR22 and cR21; (c) D29 and cD29. The x-axis represents the DUF283/hDicer molar concentrations and the y-axis the percentage content of the double-stranded fraction [f_ds]. Dashed lines are drawn for the values obtained for control experiments with BSA (baselines). For each tested pair, the maximum increase in the double-stranded fraction [Δf_ds], driven either by DUF283 or hDicer, was calculated.