Figure 7. Comparison of hDicer and Giardia Dicer annealing capacities.

Native PAGE gels showing the results of annealing reactions involving hDicer (left) or Giardia Dicer (GiDicer) (right) and the R22 and cR22 oligonucleotide pair. Reaction mixtures were incubated for 5, 15 or 30 min with ~150 nM of hDicer or with ~150 nM of GiDicer or with no protein [C-] and were then resolved in buffer containing SDS at a final concentration of 0.2%.