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. 2016 Apr 5;6:23977. doi: 10.1038/srep23977

Figure 4. The mRNA levels of cytokines in MoDCs pretreated with NF-κB inhibitor BAY11-7082 or DMSO and then stimulated with poly (I: C) and imiquimod along with inactivated PRRSV antigen.

Figure 4

MoDCs were pretreated with BAY11-7082 (5 μM) or DMSO for 2 h, then were incubated with poly (I: C) and imiquimod along with inactivated PRRSV antigen for 12 h, the inactivated PRRSV antigen and RPMI-1640 were used as control. The transcript levels of IFN-γ, IL-12 P40, IL-6 and IL-10 in MoDCs were analyzed by real-time RT-PCR. Relative transcript levels are shown as fold changes relative to the level for the control cells of the RPMI-1640 group. The data represent the means of three independent experiments, with each experiment performed in triplicate. Data represent the means ± standard deviations (error bars) of three independent experiments.