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(a) Three different topology models proposed recently for IFITM3. The hydrophobic region of IFITM3 from W60 to Y132 was analyzed using EPR methods. (b) The spin labeling reaction for cysteine substituted IFITM3 mutants to introduce the nitroxide side chain, which is denoted R1. (c) SDS-PAGE analysis of IFITM3 in detergent micelles. Both wt-IFITM3 and IFITM3-W60C were purified as monomers in detergent micelles.
