Figure 4.

Effects of the pSilence-S and -M treatments in the lethal HTNV challenge model. Suckling mice were infected with lethal doses of HTNV 76–118 and treated with pSilence-S or pSilence-M at 1 and 3 dpi, respectively. For each group, 8 mice were monitored for survival (A), 4 mice per group were sacrificed on the indicated days, and samples were collected for virological analyses (B–D). On 8 dpi, the viral loads in the lungs, kidneys and brains were determined by viral titration (B). The HTNV RNA profiles in the brains were determined with qRT-PCR on 2, 3, 4, and 5 dpi. ^bP<0.05.