Figure 2. Volcano plot of differentially regulated proteins in HEK293 cells expressing mutant (p.C240*) FOXE3.

(a) The iTRAQ protocol identified 48 differentially expressed proteins, of which 29 were downregulated and 19 upregulated in cells expressing mutant FOXE3. (b) The TMT protocol revealed a total of 31 proteins that were differentially expressed including 17 downregulated and 14 upregulated in cells expressing mutant FOXE3. Note: the fold changes are represented in log₂ scale depicted on the x-axis and the −log₁₀ p-value is depicted on the y-axis (the use of −log values means that proteins having greater statistical significance are higher in the plot). The red circles in both plots represent proteins that show 3 s.d. differential expression.