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. 2016 Mar 24;7:10953. doi: 10.1038/ncomms10953

Figure 4. In vivo modelling reveals broader developmental defects and underdeveloped cataractous lenses in dnajb1a morphants.

Figure 4

dnajb1a zebrafish morphants recapitulate PA symptoms. Dorsal view of (a) control morphant and (b) dnajb1a morphant embryos suggest a relatively compact lens observed at 4 days post fertilization as a result of dnajb1a MO. Lateral view of (c) control morphant and (d) dnajb1a morphant embryos showing broader eye development defects including underdeveloped cataractous lenses in dnajb1a MO-injected embryos. (e) Quantification of the compact lens phenotype in the dnajb1a, hspa4l and kif3a morphants and rescue of morphant phenotype with zebrafish orthologue of dnajb1a mRNA. Note: kif3a MO did not show any ocular phenotype; however, the morphants exhibited morphological and developmental defects, such as body curvature, consistent with the ciliary phenotypes. Lateral view of (f) control morphant and (g) dnajb1b morphant embryos illustrate a relatively compact cataractous lenses observed at 4 days post fertilization as a result of dnajb1b translation-blocking MO.