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. 2016 Mar 24;7:10953. doi: 10.1038/ncomms10953

Table 1. A summary of the transcriptome data of control, wild-type and truncated FOXE3-expressing cells.

Sample Sample ID Total reads (106) Total mapped reads (106) Total mapped reads w/o PCR duplication (106) % Total mapped reads w/o PCR duplication Properly paired reads (106) % properly paired reads Sequenced bases (Mb) ∼Human transcriptome coverage (×)
Lane-1: whole-transcriptome sequencing (2 × 100 bp)
 Control CNT-A1 29.62 25.50 24.50 87.72 19.32 78.87 1,932.76 29.73
  CNT-A2 34.17 29.52 28.62 83.75 22.94 80.17 2,294.60 35.30
  CNT-A3 29.39 25.33 24.60 83.72 19.93 81.00 1,993.18 30.66
  CNT-A4 30.13 26.08 25.50 84.63 20.71 81.21 2,071.26 31.86
 FOXE3 mutant MUT-A1 26.35 21.89 21.06 79.92 16.31 77.45 1,631.06 25.09
  MUT-A2 24.93 20.78 20.30 81.42 16.30 80.30 1,630.05 25.07
  MUT-A3 23.76 19.70 19.27 81.10 15.45 80.19 1,545.44 23.77
  MUT-A4 29.95 25.01 24.43 81.58 19.58 80.15 1,958.61 30.13
 FOXE3-wild type WT-A1 28.01 23.30 22.48 80.28 17.49 77.81 1,749.62 26.91
  WT-A2 29.25 24.91 24.27 82.99 19.64 80.93 1,964.89 30.22
  WT-A3 31.18 26.36 25.72 82.49 20.87 81.16 2,087.69 32.11
  WT-A4 31.35 26.52 25.81 82.33 20.94 81.16 2,094.79 32.22
 Total   348.13 294.95 286.61 82.24 229.53 80.09 22,953.99 353.13
                   
Lane-2: whole-transcriptome sequencing (2 × 100 bp)
 Control CNT-B1 31.24 26.66 25.42 81.37 19.33 76.03 1,933.31 29.74
  CNT-B2 27.57 23.58 22.95 83.23 17.86 77.86 1,786.92 27.49
  CNT-B3 28.73 24.82 24.19 84.20 19.13 79.09 1,913.35 29.43
  CNT-B4 28.91 24.90 24.24 83.84 19.10 78.79 1,910.40 29.39
 FOXE3 mutant MUT-B1 30.74 25.30 24.51 79.73 18.64 76.07 1,864.83 28.68
  MUT-B2 30.13 25.02 24.62 81.71 19.34 78.57 1,934.69 29.76
  MUT-B3 32.35 27.17 26.46 81.79 20.79 78.59 2,079.85 31.99
  MUT-B4 31.89 26.74 26.14 81.97 20.59 78.80 2,059.92 31.69
 FOXE3-wild type WT-B1 26.06 21.70 20.98 80.53 15.98 76.15 1,598.17 24.58
  WT-B2 34.50 29.08 28.43 82.40 22.51 79.21 2,251.94 34.64
  WT-B3 29.99 25.51 24.94 83.16 19.85 79.62 1,985.69 30.54
  WT-B4 32.18 27.25 26.69 82.93 21.37 80.08 2,137.50 32.88
 Total   364.35 307.79 299.91 82.23 234.56 78.24 23,456.61 360.87
Combined (lanes 1 and 2)   712.48 602.75 586.52 82.24 464.61 79.14 46,410.61 714.00

CNT, control; MUT, mutant; WT, wild type.