Figure 6. FOG-1 is required for maintenance of myeloid-biased HSCs.

(a) Representative FACS plots showing CD150 and Vwf-EGFP expression in LSK cells from Vwf-FOG-1^Con and Vwf-FOG-1^cKO transplanted recipients. Numbers show percentages relative to parental gates. (b) Percentage of EGFP⁺ cells in test cell-derived (CD45.1⁻CD45.2⁺) LSKCD150⁺ population from Vwf-FOG^Con (Con; N=10) and Vwf-FOG^cKO (cKO; N=8) transplanted mice. **P<0.01 (Student's t-test). Data are from two independent experiments. (c) Sixteen-week peripheral blood myeloid output from CD45.1⁻CD45.2⁺Lin⁻ Vwf-EGFP⁻ and Vwf-EGFP⁺ fractions isolated from Vwf-FOG-1^Con and Vwf-FOG-1^cKO primary recipients (FOG1^Con EGFP⁻: N=13; FOG1^Con EGFP⁺: N=7; FOG1^cKO EGFP⁻: N=10). The fraction of CD45.2 myeloid cells was normalized to the overall CD45.2 reconstitution. All data are mean values±s.d., after normalization to the mean value the FOG1^Con EGFP⁻ fraction. ***P<0.0001, **P<0.01 (Student's t-test). Data are from two independent experiments. (d) Sixteen-week peripheral blood lymphoid output analysed and represented as in c. (e) Sixteen-week peripheral blood platelet output analysed and represented as in c, except that significance was measured using the Mann–Whitney U-test.