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High sequence identity of 10 msg isoforms of P. jirovecii previously identified by Sanger sequencing of plasmid-cloned PCR products [22]. Text labels on the left side and the top represent plasmid ID. Colors indicate different degrees of identity from low (blue) to high (red). Plasmids containing these 10 isoforms were mixed together to form a benchmark admixture, which was amplified by PCR and sequenced in parallel by PacBio and 454 sequencing
