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. 2016 Mar 30;7:11063. doi: 10.1038/ncomms11063

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(a) Phase-contrast microscopy of RA-treated EBs differentiated into neurons 2 days after plating. (b) Tubb3 labelling of RA-treated EBs differentiated into neurons 2 days after plating. (c,d). Immunoblots on extracts from differenting ES cells and EBs with the indicated antibodies. The membranes were stained with Ponceau as loading controls. (e). Immunoblots on extracts from ES cell lines engineered to re-express recombinant Taf4. (f). Phase-contrast microscopy and Tubb3 labelling of RA-treated EBs differentiated into neurons 2 days after plating. All images were taken at × 20 magnification. Scale bars 100 μm.