Skip to main content
. 2016 Mar 30;7:11063. doi: 10.1038/ncomms11063

Figure 7. Taf4 is required for activation of neurogenic genes in differentiating ES cells.

Figure 7

(a). Gene expression changes at different stages of differentiation in Taf4a−/− compared with WT ES cells. The numbers of genes with altered expression and their fold changes at each stage are indicated. (b). Global comparison of RNA-seq data in Taf4a−/− compared to WT at the EB8 stage. The expression of several neurogenic and pluripotency genes are highlighted. (c). Comparison of the changes in expression of selected marker genes measured by RNA-seq and by RT–qPCR. (d). Kinetics of gene expression and their alterations during differentiation are represented as a clustered heatmap. The ontology terms associated with the genes in clusters 2–4 that are most affected by Taf4 loss are indicated. (e). Metaprofiles for the indicated ChIP-seq data sets corresponding to genes in clusters 2–4 are shown. For TFIIB, H3K4me3 and H3K27ac the profiles are centred on the TSS. For Pol II the profiles are show over the gene from the transcription start site (TSS) to the transcription termination site (TTS).