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. 2016 Mar 30;7:11063. doi: 10.1038/ncomms11063

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(a) Phase-contrast microscopy and Tnnt2 labelling of EBs differentiated into cardiomyocytes 9 days after plating. (b) Phase-contrast microscopy and Tnnt2 labelling of ES cells engineered to re-express exogenous Taf4 differentiated into cardiomyocytes 9 days after plating. All images were taken at × 20 magnification. (c) Global comparison of RNA-seq data in Taf4a^−/− compared with WT at the EB8 stage. The expression of several cardiomyocyte markers and pluripotency genes are highlighted. (d) Comparison of the changes in expression of selected marker genes measured by RNA-seq and by RR–qPCR. Scale bars 50 μm.