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. 2016 Mar 30;7:11056. doi: 10.1038/ncomms11056

Figure 3. Vitamin C enhances meiosis-related changes in Max expression-ablated ESCs.

Figure 3

(a) Total number of viable Dox-treated Max-null ESCs cultured in the presence or absence of vitamin C at the indicated days after Dox addition. The number of cells at day 0 (0d) was arbitrarily set to one (n=3, means±s.d.). (b) Expression levels of the Stra8 gene in Dox-treated Max-null ESCs cultured in the presence or absence of vitamin C for 4 days (4d). The expression level of the Stra8 gene in untreated control Max-null ESCs was arbitrarily set to one (n=3, means±s.d.). (c) Representative meiosis-like SYCP3-staining patterns in Dox-treated Max-null ESCs cultured in the absence or presence of vitamin C for 6 days (6d). (d) Frequency of meiosis-like SYCP3-staining patterns in Max-null ESCs treated only with Dox for 6 days or together with vitamin C.